rabbit polyclonal antibodies of anti-dkc1  (Servicebio Inc)

Journal: British Journal of Cancer

Article Title: DKC1 overexpression associated with prostate cancer progression

doi: 10.1038/sj.bjc.6605299

Figure Lengend Snippet: Expression of DKC1 in prostate cancer cell lines. ( A ) Expression of DKC1 relative to the reference gene TBP in the indicated prostate cancer cell lines and normal prostate epithelial cells (PrECs), according to quantitative RT–PCR. DKC1 and TBP were each measured in triplicate, s.e.m. are indicated. ( B ) Western blot analysis of the dyskerin protein in nuclear extracts of the indicated prostate cell lines and normal PrECs. Equal amounts of nuclear extract from each cell line were loaded and analysed for dyskerin as described in Materials and Methods.

Article Snippet: Thereafter, membranes were incubated overnight with rabbit polyclonal anti-dyskerin antibody (1 : 3000, Atlas Antibodies, Stockholm, Sweden) and mouse monoclonal anti-TBP (1 : 2000; Abcam, Cambridge, UK) as a loading control at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: British Journal of Cancer

Article Title: DKC1 overexpression associated with prostate cancer progression

doi: 10.1038/sj.bjc.6605299

Figure Lengend Snippet: Effect of DKC1 knockdown in prostate cancer cell lines. ( A ) Western blot analysis of dyskerin expression in Du145 and 22Rv1 after siRNA treatment. Top: total cell extracts; bottom: nuclear extracts; + and − symbols denote cells transfected with DKC1 siRNA and nontargeting siRNA, respectively; the slash symbol denotes untransfected cells. ( B ) Effect of siRNA on ATP amounts as an indicator of vital cell numbers after 3 days of treatment with siRNA directed against DKC1 or nontargeting siRNA (control). P -values were determined by t -test.

Article Snippet: Thereafter, membranes were incubated overnight with rabbit polyclonal anti-dyskerin antibody (1 : 3000, Atlas Antibodies, Stockholm, Sweden) and mouse monoclonal anti-TBP (1 : 2000; Abcam, Cambridge, UK) as a loading control at 4°C.

Techniques: Knockdown, Western Blot, Expressing, Transfection, Control